Genetic analysis of anemone-type and single-type inflorescences in chrysanthemum using genotyping-by-sequencing

Abstract Flower shape is a key trait of ornamental and commercial importance in breeding programs for chrysanthemum ( Chrysanthemum morifolium Ramat.). Understanding the genetic basis phenotypic variation seen inflorescence-related traits will contribute to improvement development new varieties. In this study, we investigated determinants inflorescence using an F 1 segregating population derived from cross between two cultivars with different types, ‘Puma White’ (anemone-shaped inflorescence) ‘Dancer’ (single-type inflorescence). Genotyping-by-sequencing identified 26,847 single-nucleotide polymorphisms (SNPs) 182 progenies their parents. A genome-wide association study highlighted 17 SNPs mapping 15 GBS-tags as being significantly associated three traits: flower type, number ray florets, disk diameter. No single SNP was These SNP-harboring sequences defined ten candidate genes traits. We explored transcript levels nine these buds, florets publicly available genome transcriptome data. results provide genomic foundation harness important horticultural explore avenues breeding.